Novel nested direct PCR technique for malaria diagnosis using filter paper samples.
نویسندگان
چکیده
The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.
منابع مشابه
Comparison of a Genus-specific Conventional Pcr and a Species-specific Nested-pcr for Malaria Diagnosis Using Fta Collected Samples from Kingdom of Saudi Arabia.
Molecular tools are increasingly accepted as the most sensitive and reliable techniques for malaria diagnosis and epidemiological surveys. Also, collection of finger prick blood spots onto filter papers is the most simple and affordable method for samples preservation and posterior molecular analysis, especially in rural endemic regions where malaria remains a major health problem. Two malaria ...
متن کاملValidation of microscopic diagnosis of malaria in field laboratories of malarious areas of Iran by Nested PCR
Introduction: Examination of Geimsa stained blood smears is the main method of malaria diagnosis in our country, however, accuracy of results is largely depended on the skill and laboratory conditions. This study was designed and carried out to assess the current situation and to determine the effectiveness and quality improvement of malaria diagnosis program. Methods: In this analytical s...
متن کاملA genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.
A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum-infected blood samples, it was capable of detecting six parasites/microl of blood using DNA prepar...
متن کاملDetection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers.
A modified nested polymerase chain reaction (PCR) method for detection of Plasmodium falciparum, P. vivax and P. malariae was combined with a simple blood collection and deoxyribonucleic acid (DNA) extraction method and evaluated in Malaysia. Finger-prick blood samples from 46 hospital patients and 120 individuals living in malaria endemic areas were spotted on filter papers and dried. The simp...
متن کاملLoop Mediated Isothermal Amplification (LAMP) Accurately Detects Malaria DNA from Filter Paper Blood Samples of Low Density Parasitaemias
BACKGROUND Loop mediated isothermal amplification (LAMP) provides an opportunity for improved, field-friendly detection of malaria infections in endemic areas. However data on the diagnostic accuracy of LAMP for active case detection, particularly low-density parasitaemias, are lacking. We therefore evaluated the performance of a new LAMP kit compared with PCR using DNA from filter paper blood ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 49 4 شماره
صفحات -
تاریخ انتشار 2011